Functional Analysis of Lincomycin Transporter Gene lmrC in Streptomyces Lincolnensis LC-G

Abstract

Abstract:

To investigate the role of lincomycin transporter LmrC in lincomycin biosynthesis, lmrC was deleted in a lincomycin high-yielding strain LC-G by homologous recombination. HPLC analysis and resistance assay indicated that lincomycin production and self-resistance in XJJ7, and the lmrC mutant were decreased by 55% and 50%, respectively. Quantitative real-time polymerase chain reaction(PCR) analysis showed that the transcriptional levels of structure genes lmbA and lmbR were drastically reduced, whereas the expression of a regulatory gene lmbU did not change in XJJ7. Moreover, overexpression of lmrC in LCG could not increase the lincomycin yield and the expression of lmbA, lmbR and lmbU as well. However, the resistance to lincomycin was improved in the lmrCoverexpressed strain. Therefore, lmrC was proved to be involved in lincomycin biosynthesis by affecting the transcription of structure genes and confer lincomycin resistance to Streptomyces lincolnensis.

Key words: Streptomyces lincolnensis, lincomycin, ATP-binding cassette (ABC) transporter, resistance

CLC Number:

Q 93

XU Jingjing1,WU Hang1,MENG Sitong2,LIU Ruihua3 HUANG Xunduan1,ZHANG Buchang1,BAI Linquan2. Functional Analysis of Lincomycin Transporter Gene lmrC in Streptomyces Lincolnensis LC-G[J]. Journal of Shanghai Jiaotong University, 2014, 48(02): 159-163.

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