Improvement of Immunoassay Detection Sensitivity by Using Well-Defined Raspberry-Like Magnetic Microbeads as Carriers

doi:10.1007/s12204-014-1537-z

Abstract

Abstract: Well-defined raspberry-like magnetic microbeads (RMMBs) as immunoassay solid carriers were prepared by chemical covalent binding between Fe3O4 magnetic microspheres and SiO2 nanoparticles. These RMMBs were not as agglomerative as nano-sized magnetie particles (< 200 nm), which was an advangtage for high efficient magnetic separation. When compared to Fe3O4@SiO2 core-shell magnetic microbeads (CMMBs) with smooth surface, RMMBs exhibited stronger capacity to bind biomolecules. Limit of blank (LoB) and limit of detection (LoD) of HBsAg detection using RMMBs as carriers via chemiluminiscence immunoassay (CLIA) were 0.472 and 1.022 μg/L, respectively, showing a notable improvement compared with CMMBs whose LoB and LoD were 1.017 and 1.988 μg/L, respectively. All these indicated a great potential of RMMBs in immunoassay application.

Key words: immunoassay| raspberry-like| magnetic microbeads| detection sensitivity

摘要： Well-defined raspberry-like magnetic microbeads (RMMBs) as immunoassay solid carriers were prepared by chemical covalent binding between Fe3O4 magnetic microspheres and SiO2 nanoparticles. These RMMBs were not as agglomerative as nano-sized magnetie particles (< 200 nm), which was an advangtage for high efficient magnetic separation. When compared to Fe3O4@SiO2 core-shell magnetic microbeads (CMMBs) with smooth surface, RMMBs exhibited stronger capacity to bind biomolecules. Limit of blank (LoB) and limit of detection (LoD) of HBsAg detection using RMMBs as carriers via chemiluminiscence immunoassay (CLIA) were 0.472 and 1.022 μg/L, respectively, showing a notable improvement compared with CMMBs whose LoB and LoD were 1.017 and 1.988 μg/L, respectively. All these indicated a great potential of RMMBs in immunoassay application.

关键词: immunoassay| raspberry-like| magnetic microbeads| detection sensitivity

CLC Number:

WANG Ye-fei (王叶菲), ZHANG Xin (张欣), XU Ping (徐平), GU Hong-chen (古宏晨), XU Hong* (徐宏). Improvement of Immunoassay Detection Sensitivity by Using Well-Defined Raspberry-Like Magnetic Microbeads as Carriers[J]. Journal of shanghai Jiaotong University (Science), 2014, 19(5): 538-543.

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