Biocompatibility Evaluation of Polyethylene Terephthalate Artificial Ligament Coating Hydroxyapatite by Fibroblasts Cells in Vitro

doi:10.1007/s12204-012-1352-3

Journal of shanghai Jiaotong University (Science) ›› 2012, Vol. 17 ›› Issue (6): 717-722.doi: 10.1007/s12204-012-1352-3

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Biocompatibility Evaluation of Polyethylene Terephthalate Artificial Ligament Coating Hydroxyapatite by Fibroblasts Cells in Vitro

JIANG Jia1 (蒋佳), HAO Wei2 (郝威), LI Yu-zhuo1 (李毓卓), CHEN Jun3* (陈俊),YAO Jin-rong3 (姚晋荣), SHAO Zheng-zhong2 (邵正中), Li Hong1 (李宏),YANG Jian-jun1 (杨建军), CHEN Shi-yi1* (陈世益)

(1. Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China; 2. Laboratory of Advanced Materials, National Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China; 3. Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education, Institute of Micro/Nano Science and Technology, Shanghai Jiaotong University, Shanghai 200240, China)
(1. Sports Medicine Center, Department of Sports Medicine and Arthroscopy Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China; 2. Laboratory of Advanced Materials, National Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China; 3. Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication Technology of Ministry of Education, Institute of Micro/Nano Science and Technology, Shanghai Jiaotong University, Shanghai 200240, China)

Online:2012-12-31 Published:2012-12-30
Contact: CHEN Jun3* (陈俊),CHEN Shi-yi1* (陈世益) E-mail: biochenjun@sjtu.edu.cn, chenshiyi888@163.com

Abstract

Abstract: Hydroxyapatite (HA) based materials have been widely used in the field of ligament tissue engineering in the past decades. It has been previously reported that HA can increase the penetration of marrow-derived mesenchymal stem cells (MSCs) and MSCs cells into scaffolds due to increased cell differentiation in biological media. Additionallyit was found that there are much difference between MSCs and anterior cruciate ligament (ACL) cells. For that reason, we mainly evaluate the biocompatibility of polyethylene terephthalate (PET) silk scaffold with fibroblasts cells in vitro. We cultured mouse fibroblasts cells on the substrate of PET fiber and PET-HA scaffold, respectively, and then observed the morphology by using scanning electron microscopy. Our data indicate that PET-HA scaffold has good biocompatibility with fibroblasts cells and can potentially be useful in enhancing the fibroblasts cell differentiation and proliferation.

Key words: artificial ligament| ligamentization| hydroxyapatite| fibroblasts cells| biocompatibility

摘要： Hydroxyapatite (HA) based materials have been widely used in the field of ligament tissue engineering in the past decades. It has been previously reported that HA can increase the penetration of marrow-derived mesenchymal stem cells (MSCs) and MSCs cells into scaffolds due to increased cell differentiation in biological media. Additionallyit was found that there are much difference between MSCs and anterior cruciate ligament (ACL) cells. For that reason, we mainly evaluate the biocompatibility of polyethylene terephthalate (PET) silk scaffold with fibroblasts cells in vitro. We cultured mouse fibroblasts cells on the substrate of PET fiber and PET-HA scaffold, respectively, and then observed the morphology by using scanning electron microscopy. Our data indicate that PET-HA scaffold has good biocompatibility with fibroblasts cells and can potentially be useful in enhancing the fibroblasts cell differentiation and proliferation.

关键词: artificial ligament| ligamentization| hydroxyapatite| fibroblasts cells| biocompatibility

CLC Number:

Q 81.43

JIANG Jia1 (蒋佳), HAO Wei2 (郝威), LI Yu-zhuo1 (李毓卓), CHEN Jun3* (陈俊),YAO Jin-rong3 (姚晋荣), SHAO Zheng-zhong2 (邵正中), Li Hong1 (李宏),YANG Jian-jun1 (杨建军), CHEN Shi-yi1* (陈世益). Biocompatibility Evaluation of Polyethylene Terephthalate Artificial Ligament Coating Hydroxyapatite by Fibroblasts Cells in Vitro[J]. Journal of shanghai Jiaotong University (Science), 2012, 17(6): 717-722.

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Biocompatibility Evaluation of Polyethylene Terephthalate Artificial Ligament Coating Hydroxyapatite by Fibroblasts Cells in Vitro

Biocompatibility Evaluation of Polyethylene Terephthalate Artificial Ligament Coating Hydroxyapatite by Fibroblasts Cells in Vitro

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