Journal of shanghai Jiaotong University (Science) ›› 2012, Vol. 17 ›› Issue (5): 559-566.doi: 10.1007/s12204-012-1325-6

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Interaction of Human Fibroblasts with Electrospun Composites Gelatin/PLLA, Chitosan/PLLA and PLLA Fibrous Scaffolds

Interaction of Human Fibroblasts with Electrospun Composites Gelatin/PLLA, Chitosan/PLLA and PLLA Fibrous Scaffolds

LI Jian-boa,b (李建波), HAN Junb (韩君), REN Jieb,c* (任杰)   

  1. (a. Department of Chemistry; b. School of Material Science and Engineering; c. Key laboratory Advanced Civil Engineering Materials, Ministry of Education, Tongji University, Shanghai 200092, China)
  2. (a. Department of Chemistry; b. School of Material Science and Engineering; c. Key laboratory Advanced Civil Engineering Materials, Ministry of Education, Tongji University, Shanghai 200092, China)
  • Online:2012-10-30 Published:2012-11-16
  • Contact: REN Jieb,c* (任杰) E-mail:renjie6598@163.com

Abstract: Highly porous ultrafine electrospun scaffolds, gelatin/poly(L-lactic acid) (PLLA) and chitosan/PLLA were prepared by blending gelatin and PLLA, chitosan and PLLA respectively. The biocompatibilities of these scaffolds were assessed by attachment, proliferation and viability of cells on them. The results indicated that over 30% WI-38 cells could attach to the gelatin/PLLA and chitosan/PLLA scaffolds at 2 h after seeding, while the attachment of the cells was only 15% on PLLA scaffolds. Both gelatin/PLLA and chitosan/PLLA scaffolds also exhibited a very good ability for proliferation of WI-38 cells. Cell growth on the gelatin/PLLA and chitosan/ PLLA scaffolds showed dramatic improvement, indicating a much better biocompatibility in the blends contributed by gelatin and chitosan. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay also demonstrated gelatin/PLLA showed better ability to enhance the growth and functions of the cells. These assays suggest that the electrospun gelatin/PLLA and chitosan/PLLA scaffolds are promising biomaterials with great biocompatibility for the development of skin tissue engineering.

Key words: poly(L-lactic acid) (PLLA)| gelatin| chitosan| electrospinning| fibroblast| cell culture

摘要: Highly porous ultrafine electrospun scaffolds, gelatin/poly(L-lactic acid) (PLLA) and chitosan/PLLA were prepared by blending gelatin and PLLA, chitosan and PLLA respectively. The biocompatibilities of these scaffolds were assessed by attachment, proliferation and viability of cells on them. The results indicated that over 30% WI-38 cells could attach to the gelatin/PLLA and chitosan/PLLA scaffolds at 2 h after seeding, while the attachment of the cells was only 15% on PLLA scaffolds. Both gelatin/PLLA and chitosan/PLLA scaffolds also exhibited a very good ability for proliferation of WI-38 cells. Cell growth on the gelatin/PLLA and chitosan/ PLLA scaffolds showed dramatic improvement, indicating a much better biocompatibility in the blends contributed by gelatin and chitosan. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay also demonstrated gelatin/PLLA showed better ability to enhance the growth and functions of the cells. These assays suggest that the electrospun gelatin/PLLA and chitosan/PLLA scaffolds are promising biomaterials with great biocompatibility for the development of skin tissue engineering.

关键词: poly(L-lactic acid) (PLLA)| gelatin| chitosan| electrospinning| fibroblast| cell culture

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