Abstract: Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China) Abstract： SJTD-1, a novel kind of Pseudomonas aeruginosa, is isolated from oil-contaminated soil. It has been demonstrated that this bacterium can metabolize n-alkanes (C12 to C30) in our laboratory. Pseudomonas aeruginosa SJTD-1 may have wide applications in oil pollution. In our study, phosphopeptides of Pseudomonas aeruginosa SJTD-1 were enriched by TiO2 and 13 phosphopeptides from 12 phosphoproteins were first characterized by nano-liquid chromatography-mass spectrometry (nano LC-MS). Among these identified phosphoproteins, 7 phosphoproteins were first discovered in Pseudomonas aeruginosa. We found most of these identified phosphoproteins were related to DNA replication, material transport and energy metabolism. This is the first analysis of phosphorylation proteomic in Pseudomonas aeruginosa SJTD-1, and our results provide evidence for further research on DNA replication, material or energy metabolism and membrane transport in Pseudomonas aeruginosa.

Key words: Pseudomonas aeruginosa, phosphoproteins, liquid chromatography-mass spectrometry (LC-MS)