To evaluate the biocompatibility and performance of the optimized artificial anal sphincter system, an in vivo experiment was designed. Blood was collected from the Beagle dogs’ jugular vein on days 1 and 4; at weeks 1, 2, 4 and 5, postoperatively. Biochemical parameters including total protein, albumin, total bilirubin, aspartate aminotransferase, blood urea nitrogen, creatinine, and Creactive protein were examined. The total bilirubin levels remained within the normal range, and the time courses of aspartate aminotransferase of the two Beagle dogs were within the baseline levels after 2 weeks of implantation, demonstrating no liver function complications. The blood urea nitrogen and creatinine levels remained within the normal range, indicating no renal function complications. The total protein and albumin fluctuated within the normal range throughout the duration of this study. The level of Creactive protein did not increase and no infection and inflammation were found. The experimental data indicated that the artificial anal sphincter system demonstrated no adverse influence on blood serum chemistry and exhibited an effective system performance.