上海交通大学学报

上海交通大学学报（自然版） ›› 2014, Vol. 48 ›› Issue (09): 1257-1262.

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采用图像信息学方法研究三维支架中细胞的信号通路

郭进1，TZERANIS Dimitrios S2，SO Peter T C2，魏勋斌3   

  1. (1. 复旦大学  化学系， 生物医学研究院， 上海  200433； 2.  麻省理工学院  生物工程系， 剑桥  02139， 美国； 3. 上海交通大学  MedX研究院， 上海 200030）
  • 收稿日期:2013-09-29
  • 基金资助:

    国家自然科学基金项目（30901432, 30972949）资助

Study of Signal Pathways in 3D Cell-Scaffold SystemsBased on Image Informatics Framework

GUO Jin 1,TZERANIS Dimitrios S2，SO Peter T C2,WEI Xunbin3   

  1. (1. Department of Chemistry, Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China; 2. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA; 3. MedX Research Institute, Shanghai Jiaotong University, Shanghai 200030, China)
  • Received:2013-09-29

摘要：

通过慢病毒介导的短发夹RNA对成纤维细胞的转化生长因子β（TGF-β）信号通路进行干扰，采用双光子荧光显微成像技术并结合基于细胞细胞外基质三维系统的图像信息学方法，研究了植于多孔胶原蛋白支架中成纤维细胞分化过程的TGF-β信号通路.结果表明，在介导成纤维细胞分化的过程中，生长因子TGF-β1比TGF-β3发挥的作用更大，在TGF-β1介导的成纤维细胞分化过程中，蛋白SMAD1和SMAD3通过不同方式也发挥了重要作用.所得结果与通过生化或遗传学方法的结果相吻合.

关键词:  , 图像信息学, 双光子荧光显微成像, 转化生长因子&beta, 成纤维细胞, 多孔胶原蛋白支架

Abstract:

Stable protein expression interference of key components of transforming growth factor (TGF)-β pathway in fibroblasts was achieved by transfection with a shRNA lentivirus. Two-photon microscopy, combined with an image informatics framework that suitable for 3D cellmatrix system, was used to study the TGF-β signaling in fibroblasts inside porous collagen scaffolds. It is concluded that, compared with TGF-β3, TGF-β1 plays a more important role in mediating myofibroblast differentiation, and that SMAD1 and SMAD3 play key roles in TGF-β1 mediated myofibroblast differentiation but in different ways. These conclusions were consistent with the results obtained by using biochemical or genetic methods.

Key words: image informatics, two-photon fluorescence microscopy, transforming growth factor (TGF)β, fibroblasts, porous collagen scaffolds

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