Preliminary Characteristic of Alginate, 
Heparin-Chitosan-Alginate, Heparin  Microencapsulated Hepatocytes
System

doi:10.1007/s12204-012-1238-4

Journal of shanghai Jiaotong University (Science) ›› 2012, Vol. 17 ›› Issue (1): 121-128.doi: 10.1007/s12204-012-1238-4

• Articles • Previous Articles

Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System

YU Song-lin (余松林), HAN Bao-san (韩宝三), CUI Long (崔龙), DONG Ya-dong (董亚东) , WANG Jia-xiang (王加祥), PENG Cheng-hong (彭承宏)

(Center of Organ Transplantation, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China)
(Center of Organ Transplantation, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China)

Received:2011-04-14 Online:2012-02-29 Published:2012-03-21
Contact: PENG Cheng-hong (彭承宏) E-mail:chongpengruijin@yahoo.cn

Abstract

Abstract: Abstract: Alginate, low mole mass heparin-chitosan-alginate, low mole mass heparin microcapsules (ALCAL) with good mechanical stability were made from the high voltage pulsing microcapsule shaping device. ALCAL was made of low-mole-mass heparin (LMWH), alginate (ALG) and chitosan (CS) by supramolecular layer upon layer self-assembled technique. It was found from the microscopic observation that the microcapsules had smooth surface and a porous structure with interconnected pores. The results of the permeability experiment of microcapsules using fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) and fluorescein isothiocyanate-immunoglobulin G (FITC-IgG) showed that the ALCAL membrane could provide cell immuno-isolation; meanwhile, the ALCAL membrane had good biocompatibility. The potential of ALCAL microcapsules for the encapsulation of liver cells had been investigated and showed that the ALCAL membrane supports the survival, proliferation and protein secretion on encapsulating hepatocytes. The ALCAL microcapsule had several advantages compared to more widely used alginate-chitosan-alginate (ACA) microcapsules for the application of cell therapy.

Key words: microcapsule| cell viability| hepatocytes| physical properties

摘要： Abstract: Alginate, low mole mass heparin-chitosan-alginate, low mole mass heparin microcapsules (ALCAL) with good mechanical stability were made from the high voltage pulsing microcapsule shaping device. ALCAL was made of low-mole-mass heparin (LMWH), alginate (ALG) and chitosan (CS) by supramolecular layer upon layer self-assembled technique. It was found from the microscopic observation that the microcapsules had smooth surface and a porous structure with interconnected pores. The results of the permeability experiment of microcapsules using fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) and fluorescein isothiocyanate-immunoglobulin G (FITC-IgG) showed that the ALCAL membrane could provide cell immuno-isolation; meanwhile, the ALCAL membrane had good biocompatibility. The potential of ALCAL microcapsules for the encapsulation of liver cells had been investigated and showed that the ALCAL membrane supports the survival, proliferation and protein secretion on encapsulating hepatocytes. The ALCAL microcapsule had several advantages compared to more widely used alginate-chitosan-alginate (ACA) microcapsules for the application of cell therapy.

关键词: microcapsule| cell viability| hepatocytes| physical properties

CLC Number:

YU Song-lin (余松林), HAN Bao-san (韩宝三), CUI Long (崔龙), DONG Ya-dong (董亚东) , WANG Jia-xiang (王加祥), PENG Cheng-hong (彭承宏). Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System[J]. Journal of shanghai Jiaotong University (Science), 2012, 17(1): 121-128.

References

[1] McLaughlin B E, Tosone C M, Custer L M, et al. Overview of
extracorporeal liver support systems and clinical results [J].
Annals New York Academy of Sciences, 1999, 875: 310-325.

[2] Dodson S F, Issa S, Bonham A. Liver transplantation for chronic viral
hepatitis [J]. Surgical Clinics of North America, 1999,
79(1): 131-145.

[3] Sim K H, Marinov A, Levy G A. Xenotransplantation: A potential solution
to the critical organ donor shortage [J]. Canadian Journal of
Gastroenterology, 1999, 13(4): 311-318.

[4] Mei J, Sgroi A, Mai G, et al. Improved survival of fulminant liver
failure by transplantation of microencapsulated cryopreserved
porcine hepatocytes in mice [J]. Cell Transplantation, 2009,
18(1): 101-110.

[5] Honiger J, Balladur P, Mariani P, et al. Permeability and
biocompatibility of a new hydrogel used for encapsulation of
hepatocytes [J]. Biomaterials, 1995, 16(10): 753-759.

[6] Dautzenberg H, Holzapfel G, Lukanoff B. Methods for a comprehensive
characterization of microcapsules based on polyelectrolyte complexes
[J]. Biomaterials, Artificial Cells, and
Immobilization Biotechnology, 1993, 21(3): 399-405.

[7] de Vos P, Hamel A F, Tatarkiewicz K. Considerations for successful
transplantation of encapsulated pancreatic islets [J].
Diabetologia, 2002, 45(2): 159-173.

[8] Gray D W. Encapsulated islet cells: The role of direct and indirect
presentation and the relevance to xenotransplantation and autoimmune
recurrence [J]. British Medical Bulletin, 1997, 53(4):
777-788.

[9] Betigeri S S, Neau S H. Immobilization of lipase using hydrophilic
polymers in the form of hydrogel beads [J]. Biomaterials,
2002, 23(17): 3627-3636.

[10] Pei H, Yang Y, Xi J, et al. Lineage restriction and differentiation of
human embryonic stem cells into hepatic progenitors and zone 1
hepatocytes [J]. Tissue Engineering. Part C. Methods, 2009,
15(1): 95-104.

[11] G\aaser\od O, Sannes A, Skj\aak-Br\aek G. Microcapsules of
alginate-chitosan. II. A study of capsule stability and permeability
[J]. Biomaterials, 1999, 20: 773-783.

[12] Weber M, Steinert A, Jork A, et al. Formation of cartilage matrix
proteins by BMP-transfected murine mesenchymal stem cells
encapsulated in a novel class of alginates [J]. Biomaterials,
2002, 23(9): 2003-2013.

[13] Rokstad A M, Holtan S, Strand B, et al. Microencapsulation of cells
producing therapeutic proteins: Optimizing cell growth and secretion
[J]. Cell Transplantation, 2002, 11(4): 313-324.

[14] Morris P J. Immunoprotection of therapeutic cell transplants by
encapsulation [J]. Trends in Biotechnology, 1996,
14(5): 163-167.

[15] Tyn M T, Gusek T W. Prediction of diffusion coefficients of
proteins [J]. Biotechnology and Bioengineering, 1990,
35(4): 327-338.

[16] Jones K S, Sefton M V, Gorczynski R M. In vivo recognition by the host
adaptive immune system of microencapsulated xenogeneic cells [J].
Transplantation, 2004, 78(10): 1454-1462.

[17] Goosen M F A. Physico-chemical and mass transfer considerations in
microencapsulation [J]. Annals New York Academy of Sciences,
1999, 875: 84-104.

[18] Uludag H, de Vos P, Tresco P A. Technology of mammalian cell
encapsulation [J]. Advanced Drug Delivery Reviews, 2000,
42(1): 29-64.

[19] Aoki T, Jin Z, Nishino N, et al. Intrasplenic transplantation of
encapsulated hepatocytes decreases mortality and improves liver
functions in fulminant hepatic failure from 90% partial
hepatectomy in rats [J]. Transplantation, 2005, 79(7):
783-790.

Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System

Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments