Influence of Supramolecular Chiral Hydrogel on Cellular Behavior of Endothelial Cells Under High-Glucose-Induced Injury

doi:10.1007/s12204-021-2256-x

Abstract

Abstract: In order to manage the impaired wound healing of diabetic chronic wounds induced by high-glucosedamaged endothelial cells, a glucose-induced injured endothelial cell model is established in this research to explore the regulatory effects of a supramolecular chiral hydrogel on injured endothelial cells. Cellular behaviors of endothelial cells under different culture conditions are evaluated by CCK-8, 5-ethynyl-2-deoxyuridine (EdU) staining, adhesion, and Transwell assays. The expression levels of angiogenesis-related markers, including nitric oxide,endothelial nitric oxide synthase (eNOS), and vascular endothelial growth factor (VEGF), are also assessed by enzyme-linked immune sorbent assay (ELISA) and the polymerase chain reaction (PCR). The results demonstrate that the left-handed chirality of the supramolecular chiral hydrogel can promote cell proliferation and enhance the adhesion and migration ability of impaired endothelial cells. Moreover, enhanced nitric oxide synthesis and elevated expression of eNOS and VEGF are observed in cells in the left-handed chiral environment. Thus, the left-handed supramolecular chiral hydrogel can regulate the cellular behavior of high-glucose-injured endothelial cells, including cell proliferation, adhesion, migration, and angiogenesis, offering the potential to promote diabetic wound healing.

Key words: chirality| supramolecular hydrogel| diabetes| endothelial cells| wound healing

摘要： In order to manage the impaired wound healing of diabetic chronic wounds induced by high-glucosedamaged endothelial cells, a glucose-induced injured endothelial cell model is established in this research to explore the regulatory effects of a supramolecular chiral hydrogel on injured endothelial cells. Cellular behaviors of endothelial cells under different culture conditions are evaluated by CCK-8, 5-ethynyl-2-deoxyuridine (EdU) staining, adhesion, and Transwell assays. The expression levels of angiogenesis-related markers, including nitric oxide,endothelial nitric oxide synthase (eNOS), and vascular endothelial growth factor (VEGF), are also assessed by enzyme-linked immune sorbent assay (ELISA) and the polymerase chain reaction (PCR). The results demonstrate that the left-handed chirality of the supramolecular chiral hydrogel can promote cell proliferation and enhance the adhesion and migration ability of impaired endothelial cells. Moreover, enhanced nitric oxide synthesis and elevated expression of eNOS and VEGF are observed in cells in the left-handed chiral environment. Thus, the left-handed supramolecular chiral hydrogel can regulate the cellular behavior of high-glucose-injured endothelial cells, including cell proliferation, adhesion, migration, and angiogenesis, offering the potential to promote diabetic wound healing.

关键词: chirality| supramolecular hydrogel| diabetes| endothelial cells| wound healing

CLC Number:

R 318.08

CAI Weijie (蔡伟杰), HAMUSHAN Musha (木沙·哈木山), ZHAO Changli (赵常利), CHENG Pengfei (程鹏飞), ZHONG Wanrun (钟万润), HAN Pei (韩培). Influence of Supramolecular Chiral Hydrogel on Cellular Behavior of Endothelial Cells Under High-Glucose-Induced Injury[J]. J Shanghai Jiaotong Univ Sci, 2021, 26(1): 17-24.

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