Journal of Shanghai Jiaotong University ›› 2018, Vol. 52 ›› Issue (2): 200-206.doi: 10.16183/j.cnki.jsjtu.2018.02.012

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Establishment of an In Vitro Cell Heating System Based on 3D Cell Culture

JIANG Zuomin,ZOU Jincheng,ZHANG Aili   

  1. Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200240, China
  • Online:2018-03-01 Published:2018-03-01

Abstract: To deal with the limitation of cell thermal experiment, which cannot simulate the complex micro-environment in vivo and may influence the accuracy of the experiment, the present study tried to design a petri dish which is able to heat cells both in 2D and 3D forms. At the same time, operators could constantly monitor the changes of cells during and after heating process through the microscope. Verified by experiments, this petri dish has the superiority of biocompatibility, visuality, accuracy and stability. While the 3D cell culture is used to simulate the micro-environment of the tumor in vivo, this is an effective method for cell heating and culturing.

Key words: indium tin oxides (ITO) conductive glass, thermal therapy, alginate, 3D cell culture

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