Abstract: Abstract： The influence of cell rotation on its chemical microenvironment was investigated by performing systematic numerical simulations. The influences of the line speed, rotational direction and the cell size were investigated. The results show that when a till cell starts rotating, the ambient concentration field and the flow field keep changing until they reach steady states. Different cell rotational directions lead to dramatic changes on the disturbing effect; and the peripheral speed of the cell enhances the stirring effect and hence decreases the apparent chemotactic effect.This paper contributes to the development of chemotactic experimental platform and quantitative measurement methodology.

Key words: Key words： microchannel, microfluidics, cell chemotaxis, cell rotation, concentration gradient