纳米级5-FU变形脂质体的体外透瘢痕试验

上海交通大学学报（自然版） ›› 2011, Vol. 45 ›› Issue (06): 929-934.

纳米级5-FU变形脂质体的体外透瘢痕试验

张振1，沃雁1，章一新1，毛小慧2，钱云良1，王丹茹1，贺蓉3，崔大祥3

（1. 上海交通大学医学院附属第九人民医院整形外科，上海 200011； 2. 上海交通大学医学院附属第三人民医院耳鼻喉科，上海 201900；3. 上海交通大学微纳科学技术研究院，上海 200240）

收稿日期:2010-07-05 出版日期:2011-06-29 发布日期:2011-06-29
基金资助:
上海市科委纳米技术专项基金（0852nm03000，1052nm04400），上海市科委自然科学基金（08ZR1412600）资助项目

Experimental Study of Transfersomes in Hypertrophic Scar Penetration in Vitro

ZHANG Zhen-1, WO Yan-1, ZHANG Yi-Xin-1, MAO Xiao-Hui-2, QIAN Yun-Liang-1, WANG Dan-Ru-1, HE Rong-3, CUI Da-Xiang-3

(1.Department of Plastic and Reconstructive Surgery, the Ninth Peoples Hospital, Shanghai 200021, China; 2. Department of Otorhinolaryngology Head and Neck Surgery, the Third People's Hospital, Affiliated to Shanghai Jiaotong University Shanghai 201900, China; 3. Institute of Micro-Nano Science and Technology, Shanghai Jiaotong University, Shanghai 200240, China)

Received:2010-07-05 Online:2011-06-29 Published:2011-06-29

摘要/Abstract

摘要： 为了研究变形脂质体在增生性瘢痕中的渗透作用，制备载抗瘢痕药物5-氟尿嘧啶（5-FU）的纳米级变形脂质体，用荧光剂罗丹明6GO标记，进行体外透皮、透瘢痕试验，通过高效液相色谱分析检测不同作用时间后皮肤和增生性瘢痕的累积药物透过量以及24 h后皮肤和瘢痕组织内的药物滞留量，并采用激光共聚焦显微镜分析荧光强度.各组24 h药物累积透过量由大至小的排序为：5-FU变形脂质体透瘢痕组（T-scar）、5FU水溶液透瘢痕组（C-scar）、5-FU变形脂质体透皮肤组（T-skin）、5-FU水溶液透皮肤组（C-skin）；24 h后皮肤和瘢痕组织内的药物滞留量以及荧光强度由大至小的排序为：T-scar、T-skin、C-scar、C-skin.结果表明：变形脂质体在增生性瘢痕中的渗透能力最强，对于增生性瘢痕来说，变形脂质体是一种高效的载药渗透材料.

关键词: 变形脂质体, 5-氟尿嘧啶, 增生性瘢痕, 经皮给药

Abstract: Hypertrophic scar has its special tissue structures. Drug penetration in the local is the key role of topical effect. The purpose of this study is to characterize a novel transdermal delivery carrier: transfersomes containing 5fluorouracil (5-FU) to investigate the delivery of drugs from transfersomes in human hypertrophic scar in vitro. Cumulative 5FU that permeated hypertrophic scar and skin after 24 h is: transfersomes via hypertrophic scar (T-Scar) > 5-FU solution via hypertrophic scar (CScar) > transfersomes via skin (T-Skin) > 5-FU solution via skin (C-Skin). The depositions of 5FU and intensities of fluorescence after application for 24 hours are: T-scar> T-skin> C-scar> C-skin. Conclusively, the delivery ability of transfersomes is most efficient in human hypertrophic scar. The test results indicate that transfersome is a high efficient carrier in hypertrophic scar.

Key words: transfersome, 5-fluorouracil(5-FU), hypertrophic scar, transdermal delivery

中图分类号:

张振1, 沃雁1, 章一新1, 毛小慧2, 钱云良1, 王丹茹1, 贺蓉3, 崔大祥3. 纳米级5-FU变形脂质体的体外透瘢痕试验[J]. 上海交通大学学报（自然版）, 2011, 45(06): 929-934.

ZHANG Zhen-1, WO Yan-1, ZHANG Yi-Xin-1, MAO Xiao-Hui-2, QIAN Yun-Liang-1, WANG Dan-Ru-1, HE Rong-3, CUI Da-Xiang-3. Experimental Study of Transfersomes in Hypertrophic Scar Penetration in Vitro[J]. Journal of Shanghai Jiaotong University, 2011, 45(06): 929-934.

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