Medicine-Engineering Interdisciplinary Research

Gene Expression Profiling Identifies Potential Biomarkers for Colorectal Cancer Using NanoString nCounter Assay

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  • (Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China)

Received date: 2021-08-26

  Accepted date: 2021-10-18

  Online published: 2023-07-31

Abstract

Colorectal cancer (CRC) is one of the most common malignancies worldwide. Development of predictivemolecular markers may help to achieve the best outcome in clinic. The purpose of this study is to identify thedifferentially expressed genes and new potential predictive and prognostic molecular biomarkers for CRC. Inthis study, CRC and matched normal tissues acquired from the same patient were used to extract total RNA.The NanoString nCounter assay was applied to determine the differentially expressed genes. The results werethen validated by using the Cancer Genome Atlas data. Finally, we identified 27 genes that revealed significantcorrelation with CRC in the tumor tissue. Several genes in the pan-cancer panel showed significant differentialexpression, which were more universal than others in the CRC tissue. Since some of them have not been reportedas being directly related to CRC yet, future mechanism studies can be designed based on this study. Our studydemonstrated NanoString nCounter assay could serve as an alternative approach for gene expression analysis andidentified several unreported differently expressed genes in CRC patients, which may provide some importantclues for more in-depth study of CRC and serve as potential predictive molecular biomarkers for clinical diagnosisapplication.

Cite this article

ZHAO Hui (赵晖),WEN Baiqing (文柏清),KANG Yani*(康亚妮) . Gene Expression Profiling Identifies Potential Biomarkers for Colorectal Cancer Using NanoString nCounter Assay[J]. Journal of Shanghai Jiaotong University(Science), 2023 , 28(4) : 432 . DOI: 10.1007/s12204-022-2440-7

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