We present a novel fluorescence spectral unmixing based on target-to-background separation preprocessing,
which effectively separates the multi-target fluorescence from all background autofluorescence (BF)
without any hardware-based BF acquisition and tissue specific BF estimation. Specifically, we first enhance the
intrinsic accumulation contrast in target-to-background fluorescence using h-dome transformation; then separate
multi-target fluorescence areas from the background in sparse multispectral data utilizing kernel maximum autocorrelation
factor analysis; we further use fast marching-based image inpainting method to patch up the removed
target fluorescence areas and reconstruct the multispectral BF; with the BF matrix being subtracted from the
original data, the multi-target fluorophores are easily unmixed from the subtracted data using multivariate curve
resolution-alternating least squares method. In two preliminary in-vivo experiments, the proposed method demonstrated
excellent performance to unmix multi-target fluorescences while other state-of-art unmixing methods failed
to get desired results.
ZHAO Yong (赵勇), HU Cheng (胡程), PENG Jin-liang*(彭金良), QIN Bin-jie* (秦斌杰)
. Target-to-Background Separation for Spectral Unmixing in In-Vivo Fluorescence Imaging[J]. Journal of Shanghai Jiaotong University(Science), 2014
, 19(5)
: 600
-611
.
DOI: 10.1007/s12204-014-1548-9
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