Fungal infection remains a major problem worldwide, yet treatment options are limited owing to the
lack of effective drugs, the significant toxicity of available compounds, and the emergence of drug resistance. The
low toxicity of calcofluor white (CFW) is an attractive antifungal compound for its known inhibitive effects on
trichophyton rubrum and candida albicans growth. However, the efficacy of CFW is limited in most cases. In
order to search for effective means to improve its efficacy, using saccharomyces cerevisiae as a model, we have
used microarrays to examine the cell’s response when treated with CFW on the genome scale. We found that
both the PKC-SLT2 (i.e, protein kinase C-mitogen activated protein kinase) and the glycogen metabolic pathways
are activated upon CFW treatment. These results suggest that the key components in these pathways could be
targeted by other drugs to counter the cell’s compensative response, thus to further substantiate the inhibitive
effect of CFW on fungal growth, which may lead to treatment regimens with improved efficacy of this compound
in clinical applications.
ZHOU Juana(周娟), HU Chuan-shengb (胡传圣), LI Xiao-lina (李晓林),CHENG Mingb (程酩), GUO Yanb* (郭妍), SHAO Zhi-fengb (邵志峰)
. Genome-Wide Profiling Identifies Saccharomyces Cerevisiae Response in PKC-SLT2 Signaling and Glycogen Metabolic Pathways to Antifungal Compound Calcofluor White[J]. Journal of Shanghai Jiaotong University(Science), 2014
, 19(5)
: 574
-579
.
DOI: 10.1007/s12204-014-1544-0
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