Cyclooxygenase-2 (COX-2), an important enzyme, plays a pathological role in diseases, which can
be inhibited by proanthocyanidins (PCs) effectively. In this paper, we investigated the inhibitive mechanism of
COX-2 performed by PCs. Reverse transcription-polymerase chain reaction (RT-PCR) was performed to identify
the mRNA expression level of COX-2 in A549 cell, which was induced by interleukin-1 beta (IL-1β). The pGL3
luciferase reporter vector containing the COX-2 gene promoter fragment (pGL3/COX-2p) was transfected into
A549 cell induced by IL-1β, the interference on the COX-2 promoter activity from PCs was analyzed using a dualluciferase
reporter assay, and the expressions of the nuclear factor κB composed of subunit p65 (NF-κB/p65) and
the inhibitor-κB (I-κB) were measured by the Western blotting and immunocytochemistry. The results exhibited
that PCs not only inhibited the transcript of COX-2 mRNA and the COX-2 promoter activity, but also suppressed
the nuclear translocation of NF-κB/p65 protein and the degradation of I-κB protein.
Key words: cyclooxygenase-2 (COX-2)| proanthocyanidins (PCs)| nuclear factor κB/p65 (NF-κB/p65)| inhibitor-κB (I-κB)
LU Ting-ting1 (卢婷婷), LIANG Tong2 (梁统), ZHAO Yu-cong1 (赵玉丛), ZHOU Ke-yuan2 (周克元)
. Inhibitive Effect of Proanthocyanidins on Cyclooxygenase-2
Expression in A549 Cells Induced by Cytokine Interleukin-1 Beta[J]. Journal of Shanghai Jiaotong University(Science), 2012
, 17(4)
: 500
-504
.
DOI: 10.1007/s12204-012-1312-y
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