3D Printing Bioink Preparation and Application in Cartilage Tissue Reconstruction in Vitro

doi:10.1007/s12204-021-2292-6

J Shanghai Jiaotong Univ Sci ›› 2021, Vol. 26 ›› Issue (3): 267-271.doi: 10.1007/s12204-021-2292-6

3D Printing Bioink Preparation and Application in Cartilage Tissue Reconstruction in Vitro

SUN Binbin‡ (孙彬彬), HAN Yu‡ (韩煜), JIANG Wenbo (姜闻博), DAI Kerong(戴尅戎)

(Department of Orthopaedic Surgery, Shanghai Key Laboratory of Orthopaedic Implants; Clinical and
Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital,
Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China)

出版日期:2021-06-28 发布日期:2021-06-02
通讯作者: JIANG Wenbo (姜闻博), DAI Kerong(戴尅戎) E-mail:jwb 3dprinting@163.com, krdai@163.com
基金资助:
the National Key Research and Development
Program of China (Nos. 2018YFB1105600, and
2018YFA0703000), the National Natural Science Foundation
of China (No. 81802131), and the China Postdoctoral
Science Foundation (No. 2019T120347)

3D Printing Bioink Preparation and Application in Cartilage Tissue Reconstruction in Vitro

SUN Binbin‡ (孙彬彬), HAN Yu‡ (韩煜), JIANG Wenbo (姜闻博), DAI Kerong(戴尅戎)

(Department of Orthopaedic Surgery, Shanghai Key Laboratory of Orthopaedic Implants; Clinical and
Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital,
Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China)

Online:2021-06-28 Published:2021-06-02
Contact: JIANG Wenbo (姜闻博), DAI Kerong(戴尅戎) E-mail:jwb 3dprinting@163.com, krdai@163.com
Supported by:
the National Key Research and Development
Program of China (Nos. 2018YFB1105600, and
2018YFA0703000), the National Natural Science Foundation
of China (No. 81802131), and the China Postdoctoral
Science Foundation (No. 2019T120347)

摘要/Abstract

摘要： Three-dimensional (3D) bioprinting technology has great potential for application in the treatment of cartilage defects. However, the preparation of biocompatible and stable bioinks is still a major challenge. In this study, decellularized extracellular matrix (dECM) of soft tissue was used as the basic material to prepare the bioink. Our results showed that this novel dECM-derived bioink had good printing performance and comprised a large number of fine nanofibers. Biological characterization revealed that the bioink was compatible with the growth of chondrocytes and that the nanofibrous structure greatly promoted cell proliferation. Histological and immunohistochemical analyses showed that the in vitro printed cartilage displayed the presence of characteristic cartilage lacunae. Thus, a new preparation method for dECM-derived bioink with potential application in generation of cartilage was developed in this study.

Abstract: Three-dimensional (3D) bioprinting technology has great potential for application in the treatment of cartilage defects. However, the preparation of biocompatible and stable bioinks is still a major challenge. In this study, decellularized extracellular matrix (dECM) of soft tissue was used as the basic material to prepare the bioink. Our results showed that this novel dECM-derived bioink had good printing performance and comprised a large number of fine nanofibers. Biological characterization revealed that the bioink was compatible with the growth of chondrocytes and that the nanofibrous structure greatly promoted cell proliferation. Histological and immunohistochemical analyses showed that the in vitro printed cartilage displayed the presence of characteristic cartilage lacunae. Thus, a new preparation method for dECM-derived bioink with potential application in generation of cartilage was developed in this study.

中图分类号:

R 318.08

SUN Binbin (孙彬彬), HAN Yu (韩煜), JIANG Wenbo (姜闻博), DAI Kerong(戴尅戎). 3D Printing Bioink Preparation and Application in Cartilage Tissue Reconstruction in Vitro[J]. J Shanghai Jiaotong Univ Sci, 2021, 26(3): 267-271.

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3D Printing Bioink Preparation and Application in Cartilage Tissue Reconstruction in Vitro

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