Preliminary Characteristic of Alginate, 
Heparin-Chitosan-Alginate, Heparin  Microencapsulated Hepatocytes
System

doi:10.1007/s12204-012-1238-4

上海交通大学学报（英文版） ›› 2012, Vol. 17 ›› Issue (1): 121-128.doi: 10.1007/s12204-012-1238-4

• 论文 • 上一篇

Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System

YU Song-lin (余松林), HAN Bao-san (韩宝三), CUI Long (崔龙), DONG Ya-dong (董亚东) , WANG Jia-xiang (王加祥), PENG Cheng-hong (彭承宏)

(Center of Organ Transplantation, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China)

收稿日期:2011-04-14 出版日期:2012-02-29 发布日期:2012-03-21
通讯作者: PENG Cheng-hong (彭承宏) E-mail:chongpengruijin@yahoo.cn

Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System

YU Song-lin (余松林), HAN Bao-san (韩宝三), CUI Long (崔龙), DONG Ya-dong (董亚东) , WANG Jia-xiang (王加祥), PENG Cheng-hong (彭承宏)

(Center of Organ Transplantation, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China)

Received:2011-04-14 Online:2012-02-29 Published:2012-03-21
Contact: PENG Cheng-hong (彭承宏) E-mail:chongpengruijin@yahoo.cn

摘要/Abstract

摘要： Abstract: Alginate, low mole mass heparin-chitosan-alginate, low mole mass heparin microcapsules (ALCAL) with good mechanical stability were made from the high voltage pulsing microcapsule shaping device. ALCAL was made of low-mole-mass heparin (LMWH), alginate (ALG) and chitosan (CS) by supramolecular layer upon layer self-assembled technique. It was found from the microscopic observation that the microcapsules had smooth surface and a porous structure with interconnected pores. The results of the permeability experiment of microcapsules using fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) and fluorescein isothiocyanate-immunoglobulin G (FITC-IgG) showed that the ALCAL membrane could provide cell immuno-isolation; meanwhile, the ALCAL membrane had good biocompatibility. The potential of ALCAL microcapsules for the encapsulation of liver cells had been investigated and showed that the ALCAL membrane supports the survival, proliferation and protein secretion on encapsulating hepatocytes. The ALCAL microcapsule had several advantages compared to more widely used alginate-chitosan-alginate (ACA) microcapsules for the application of cell therapy.

关键词: microcapsule, cell viability, hepatocytes, physical properties

Abstract: Abstract: Alginate, low mole mass heparin-chitosan-alginate, low mole mass heparin microcapsules (ALCAL) with good mechanical stability were made from the high voltage pulsing microcapsule shaping device. ALCAL was made of low-mole-mass heparin (LMWH), alginate (ALG) and chitosan (CS) by supramolecular layer upon layer self-assembled technique. It was found from the microscopic observation that the microcapsules had smooth surface and a porous structure with interconnected pores. The results of the permeability experiment of microcapsules using fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) and fluorescein isothiocyanate-immunoglobulin G (FITC-IgG) showed that the ALCAL membrane could provide cell immuno-isolation; meanwhile, the ALCAL membrane had good biocompatibility. The potential of ALCAL microcapsules for the encapsulation of liver cells had been investigated and showed that the ALCAL membrane supports the survival, proliferation and protein secretion on encapsulating hepatocytes. The ALCAL microcapsule had several advantages compared to more widely used alginate-chitosan-alginate (ACA) microcapsules for the application of cell therapy.

Key words: microcapsule, cell viability, hepatocytes, physical properties

中图分类号:

YU Song-lin (余松林), HAN Bao-san (韩宝三), CUI Long (崔龙), DONG Ya-dong (. Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System[J]. 上海交通大学学报（英文版）, 2012, 17(1): 121-128.

YU Song-lin (余松林), HAN Bao-san (韩宝三), CUI Long (崔龙), DONG Ya-dong (董亚东) , WANG Jia-xiang (王加祥), PENG Cheng-hong (彭承宏). Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System[J]. Journal of shanghai Jiaotong University (Science), 2012, 17(1): 121-128.

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Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System

Preliminary Characteristic of Alginate, Heparin-Chitosan-Alginate, Heparin Microencapsulated Hepatocytes System

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