Genome-Wide Profiling Identifies Saccharomyces Cerevisiae Response in PKC-SLT2 Signaling and Glycogen Metabolic Pathways to Antifungal Compound Calcofluor White

doi:10.1007/s12204-014-1544-0

上海交通大学学报（英文版） ›› 2014, Vol. 19 ›› Issue (5): 574-579.doi: 10.1007/s12204-014-1544-0

Genome-Wide Profiling Identifies Saccharomyces Cerevisiae Response in PKC-SLT2 Signaling and Glycogen Metabolic Pathways to Antifungal Compound Calcofluor White

ZHOU Juana(周娟), HU Chuan-shengb (胡传圣), LI Xiao-lina (李晓林),CHENG Mingb (程酩), GUO Yanb* (郭妍), SHAO Zhi-fengb (邵志峰)

(a. Institute of System Biomedicine; b. Bio-ID Center, School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai 200240, China)

出版日期:2014-10-31 发布日期:2014-11-12
通讯作者: GUO Yan (郭妍) E-mail: yanguo@sjtu.edu.cn

Genome-Wide Profiling Identifies Saccharomyces Cerevisiae Response in PKC-SLT2 Signaling and Glycogen Metabolic Pathways to Antifungal Compound Calcofluor White

ZHOU Juana(周娟), HU Chuan-shengb (胡传圣), LI Xiao-lina (李晓林),CHENG Mingb (程酩), GUO Yanb* (郭妍), SHAO Zhi-fengb (邵志峰)

(a. Institute of System Biomedicine; b. Bio-ID Center, School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai 200240, China)

Online:2014-10-31 Published:2014-11-12
Contact: GUO Yan (郭妍) E-mail: yanguo@sjtu.edu.cn

摘要/Abstract

摘要： Fungal infection remains a major problem worldwide, yet treatment options are limited owing to the lack of effective drugs, the significant toxicity of available compounds, and the emergence of drug resistance. The low toxicity of calcofluor white (CFW) is an attractive antifungal compound for its known inhibitive effects on trichophyton rubrum and candida albicans growth. However, the efficacy of CFW is limited in most cases. In order to search for effective means to improve its efficacy, using saccharomyces cerevisiae as a model, we have used microarrays to examine the cell’s response when treated with CFW on the genome scale. We found that both the PKC-SLT2 (i.e, protein kinase C-mitogen activated protein kinase) and the glycogen metabolic pathways are activated upon CFW treatment. These results suggest that the key components in these pathways could be targeted by other drugs to counter the cell’s compensative response, thus to further substantiate the inhibitive effect of CFW on fungal growth, which may lead to treatment regimens with improved efficacy of this compound in clinical applications.

关键词: saccharomyces cerevisiae, antifungal, calcofluor white, gene expression, signaling pathway

Abstract: Fungal infection remains a major problem worldwide, yet treatment options are limited owing to the lack of effective drugs, the significant toxicity of available compounds, and the emergence of drug resistance. The low toxicity of calcofluor white (CFW) is an attractive antifungal compound for its known inhibitive effects on trichophyton rubrum and candida albicans growth. However, the efficacy of CFW is limited in most cases. In order to search for effective means to improve its efficacy, using saccharomyces cerevisiae as a model, we have used microarrays to examine the cell’s response when treated with CFW on the genome scale. We found that both the PKC-SLT2 (i.e, protein kinase C-mitogen activated protein kinase) and the glycogen metabolic pathways are activated upon CFW treatment. These results suggest that the key components in these pathways could be targeted by other drugs to counter the cell’s compensative response, thus to further substantiate the inhibitive effect of CFW on fungal growth, which may lead to treatment regimens with improved efficacy of this compound in clinical applications.

Key words: saccharomyces cerevisiae, antifungal, calcofluor white, gene expression, signaling pathway

中图分类号:

Q 291

ZHOU Juana(周娟), HU Chuan-shengb (胡传圣), LI Xiao-lina (李晓林),CHENG Mingb (程酩), GUO . Genome-Wide Profiling Identifies Saccharomyces Cerevisiae Response in PKC-SLT2 Signaling and Glycogen Metabolic Pathways to Antifungal Compound Calcofluor White [J]. 上海交通大学学报（英文版）, 2014, 19(5): 574-579.

ZHOU Juana(周娟), HU Chuan-shengb (胡传圣), LI Xiao-lina (李晓林),CHENG Mingb (程酩), GUO Yanb* (郭妍), SHAO Zhi-fengb (邵志峰). Genome-Wide Profiling Identifies Saccharomyces Cerevisiae Response in PKC-SLT2 Signaling and Glycogen Metabolic Pathways to Antifungal Compound Calcofluor White [J]. Journal of shanghai Jiaotong University (Science), 2014, 19(5): 574-579.

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Genome-Wide Profiling Identifies Saccharomyces Cerevisiae Response in PKC-SLT2 Signaling and Glycogen Metabolic Pathways to Antifungal Compound Calcofluor White

Genome-Wide Profiling Identifies Saccharomyces Cerevisiae Response in PKC-SLT2 Signaling and Glycogen Metabolic Pathways to Antifungal Compound Calcofluor White

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