上海交通大学学报（英文版） ›› 2014, Vol. 19 ›› Issue (5): 565-568.doi: 10.1007/s12204-014-1542-2

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Direct Resolution of the Pitch of DNA on Positively Charged Lipid Bilayers by Frequency-Modulation AFM

ZHANG Jin-jin1 (张金金), LV Jun-hong1 (吕军鸿), SUN Jie-lin2,3 (孙洁林), HU Jun1 (胡钧),CZAJKOWSKY Daniel M2*, SHEN Yi2* (沈轶)   

  1. (1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; 2. School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai 200240, China; 3. Key Laboratory of Systems Biomedicine, Shanghai Jiaotong University, Shanghai 200240, China)
  • 出版日期:2014-10-31 发布日期:2014-11-12
  • 通讯作者: CZAJKOWSKY Daniel M, SHEN Yi (沈轶) E-mail:dczaj@sjtu.edu.cn, shenyi12@sjtu.edu.cn

Direct Resolution of the Pitch of DNA on Positively Charged Lipid Bilayers by Frequency-Modulation AFM

ZHANG Jin-jin1 (张金金), LV Jun-hong1 (吕军鸿), SUN Jie-lin2,3 (孙洁林), HU Jun1 (胡钧),CZAJKOWSKY Daniel M2*, SHEN Yi2* (沈轶)   

  1. (1. Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; 2. School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai 200240, China; 3. Key Laboratory of Systems Biomedicine, Shanghai Jiaotong University, Shanghai 200240, China)
  • Online:2014-10-31 Published:2014-11-12
  • Contact: CZAJKOWSKY Daniel M, SHEN Yi (沈轶) E-mail:dczaj@sjtu.edu.cn, shenyi12@sjtu.edu.cn

摘要： High resolution structural studies of DNA and DNA binding proteins by atomic force microscopy (AFM) require well-bound samples on suitably flat substrates. Adsorbing the DNA onto a positively charged supported lipid bilayer has previously been shown to be a potentially effective strategy for structural studies with AFM. Here, using our home-built frequency-modulation AFM (FM-AFM), we show that these bilayer substrates are only maximally effective for high resolution AFM when the samples are short, linear DNA, compared with circular plasmid DNA. We find that, with the former sample, the measured width of the DNA is about 2 nm, the known DNA diameter, and there is a clear height modulation along the length of the DNA with a periodicity of about 3.4 nm, in excellent agreement with the known pitch of the double helix. This sample preparation strategy is expected to enable higher resolution studies of DNA and DNA binding proteins with FM-AFM than that can presently be achieved.

关键词: frequency-modulation atomic force microscopy (FM-AFM), DNA, double helix, high resolution

Abstract: High resolution structural studies of DNA and DNA binding proteins by atomic force microscopy (AFM) require well-bound samples on suitably flat substrates. Adsorbing the DNA onto a positively charged supported lipid bilayer has previously been shown to be a potentially effective strategy for structural studies with AFM. Here, using our home-built frequency-modulation AFM (FM-AFM), we show that these bilayer substrates are only maximally effective for high resolution AFM when the samples are short, linear DNA, compared with circular plasmid DNA. We find that, with the former sample, the measured width of the DNA is about 2 nm, the known DNA diameter, and there is a clear height modulation along the length of the DNA with a periodicity of about 3.4 nm, in excellent agreement with the known pitch of the double helix. This sample preparation strategy is expected to enable higher resolution studies of DNA and DNA binding proteins with FM-AFM than that can presently be achieved.

Key words: frequency-modulation atomic force microscopy (FM-AFM), DNA, double helix, high resolution

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