Journal of Shanghai Jiao Tong University

Journal of Shanghai Jiaotong University >

2023 , Vol. 57 >Issue 9: 1196 - 1202

DOI: https://doi.org/10.16183/j.cnki.jsjtu.2022.361

Electronic Information and Electrical Engineering

Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature Control

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  • School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

Received date: 2022-09-14

  Revised date: 2022-11-14

  Accepted date: 2022-11-16

  Online published: 2023-09-27

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Abstract

The thermal cycling system is the key component of the real-time fluorescent polymerase chain reaction (PCR) instrument, which determines the nucleic acid detection efficiency and result accuracy. Aiming at the problems that the thermal cycle of the traditional PCR detection system takes a long time and the temperature control is complicated, a real-time fluorescent PCR thermal cycle system with partition temperature control is designed, including the hardware circuit and mechanical structure of the thermal cycle system. The rapid thermal cycling is achieved by switching the test solution between different constant temperature zones, and the temperature is controlled using an incremental proportional integral derivative (PID) algorithm, with a control precision of ±0.1 ℃. Using Fluent software to establish heat transfer model, the thermal delay phenomenon of the test solution was analyzed to predict the temperature variation pattern of the test solution. A prototype is built for testing and verification, and the heating and cooling rates of the test liquid are 3.8 ℃/s and 4.4 ℃/s. It is verified that the proposed PCR thermal cycling system can effectively improve the detection efficiency.

Key words： polymerase chain reaction (PCR) apparatus; temperature control; proportional integral derivative (PID) control; finite element method; heat transfer mechanism

Cite this article

CHEN Erdong, GAO Zihang, WANG Kundong, LEI Huaming . Design of a Rapid Thermal Cycling System for Real-Time Fluorescent PCR with Zone Temperature Control[J]. Journal of Shanghai Jiaotong University, 2023 , 57(9) : 1196 -1202 . DOI: 10.16183/j.cnki.jsjtu.2022.361

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