A new type host-guest structured bead-on-bead (BOB) particle was designed and synthesized via covalent assembling guest nano silica particles onto host magnetic polymer particles. Using BOB particles as biocarrier, anti-human chorionic gonadotrophin (hCG) antibody was covalently immobilized on the surface of BOB particles. Then, the immunofluorescent detection capability in recognition hCG antigen on flow cytometry was studied and compared with traditional enzyme-linked immuno sorbent assay (ELISA). The result showed that BOB particles presented remarkable immunofluorescent detection capability on flow cytometry. BOB particles also showed excellent linear detection than traditional ELISA detection in extremely low particles amount. Further more, the limit of blank (LOB) of immunofluorescent detection on flow cytometry was nearly 1/7 to traditional ELISA detection.
WU Zhimin,XU Hong,GU Hongchen
. Research on Immunofluorescent Detection Method by Flow Cytometry Based on Bead-on-Bead Structured Particles[J]. Journal of Shanghai Jiaotong University, 2017
, 51(11)
: 1361
-1366
.
DOI: 10.16183/j.cnki.jsjtu.2017.11.012
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