Lytic Effect of Environmental Factors on Algae-lysing Bacteria Strain S7 by Fluorescence Spectroscopy

Abstract

Abstract: Anabaena flos-aquae were scanned in vivo by fluorescence spectrum scanning to acquire characteristic fluorescence wavelength of phycocyanin and build a linear relationship between Anabaena flosaquae biomass and the phycocyanin characteristic fluorescence intensity. With the change of cyanobacteria growth conditions, the lytic effects of varying environmental factors (temperature, illumination and pH) to the algaelysing bacterial strain S7 were studied. The results show that utilizing fluorescence spectrum for detecting the phycocyanin fluorescence intensity and reflecting anabaena flosaquae biomass are simple, fast, accurate and feasible. Environmental factors affect the lytic effect of algaelysing bacterial strain S7. Levels of lytic effects are 30 °C>35 °C>40 °C>25 °C, dark＞light cycle＞full light, pH 5 >pH 10>pH 6>pH 9>pH 8>pH 7. For utilizing algicidal bacteria to control water bloom, exploring optimal environmental conditions for restraining the growth of those bacteria is of practical significance.
Key words：

Key words: fluorescence spectrometry, Anabaena flosaquae, algicidal bacteria, phycocyanin, environmental factor

CLC Number:

X 172

WANG Jin-Xia, LUO Gu-Yuan, XU Xiao-Yi, YE Jiang-Yu. Lytic Effect of Environmental Factors on Algae-lysing Bacteria Strain S7 by Fluorescence Spectroscopy[J]. Journal of Shanghai Jiaotong University, 2012, 46(05): 780-784.

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