Drosophila melanogaster has been a popular model organism in the study of sleep and circadian rhythm. The Drosophila activity monitoring (DAM) system is one of the many tools developed for investigating sleep behavior in fruit flies and has been acknowledged by researchers around the world for its simplicity and cost-effectiveness. Based on the simple activity data collected by the DAM system, a wide range of parameters can be generated for sleep and circadian studies. However, current programs that analyze DAM data cover a limited number of metrics and fail to provide individual data for the user to plot graphs and conduct analysis using other software. Therefore, we have developed SleepyFlyR, an R package that: (1) is simple and easy to use with a user-friendly user interface script; (2) provides a comprehensive analysis of sleep and activity parameters; (3) generates double-plotted graphs for sleep and activity patterns; (4) offers visualization of sleep and activity profiles across multiple days or within a single day; (5) calculates the changes of sleep and activity parameters between baseline and experiment; (6) stores both summary data and individual data in files with unique title.
Mou Yang, Ping Yong
. SleepyFlyR: An R Package for Sleep and Activity Analysis in Drosophila[J]. Journal of Shanghai Jiaotong University(Science), 2025
, 30(4)
: 744
-750
.
DOI: 10.1007/s12204-024-2706-3
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