In the two-step vitamin C fermentation process, its precursor 2-keto-L-gulonic acid was synthesized
from L-sorbose by mixed culture of Gluconobacter oxydans and Bacillus megaterium. The interaction between
Gluconobacter oxydans and Bacillus megaterium remains unclear and it is a challenge to mathematically model the
mixed growth of these two strains. The Monod-type equations were previously proposed to describe the coupled
growth of Gluconobacter oxydans and Bacillus megaterium. However, in this study, we modeled the interaction of
these two strains in a macroscopic view by introducing the population theory. Taking account of the fact that the
density or concentration of Gluconobacter oxydans or Bacillus megaterium was hardly to measure accurately in the
mixed culture broth, the data of concentrations of the substrate and product were used to indirectly investigate the
relation between these two strains. Three batch experiments were used to validate our model. And according to the
values of identified parameters, the type of interaction between Gluconobacter oxydans and Bacillus megaterium
was concluded to be predation, where Gluconobacter oxydans was predator, and Bacillus megaterium was prey.
ZHANG Zhi-xiong1 (张志雄), SUN Jun-wei2 (孙君伟), YUAN Jing-qi1* (袁景淇)
. Investigating the Interaction Between Gluconobacter Oxydans and Bacillus Megaterium for 2-keto-L-gulonic Acid Biosynthesis in the Two-Step Vitamin C Fermentation[J]. Journal of Shanghai Jiaotong University(Science), 2015
, 20(3)
: 281
-285
.
DOI: 10.1007/s12204-015-1622-y
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