J Shanghai Jiaotong Univ Sci

Journal of Shanghai Jiaotong University(Science) >

2011 , Vol. 16 >Issue 5: 628 - 635

DOI: https://doi.org/10.1007/s12204-011-1200-x

Articles

Comparison of Osteogenesis Between Two Kinds of Stem Cells from Goat
Combined Calcium Phosphate Cement in Tissue Engineering

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  • (1. Department of Stomatology, the Sixth People's
    Hospital Affiliated to Shanghai Jiaotong University, Shanghai
    200233, China; 2. Oral Bioengineering Laboratory/Oral Tissue Engineering
    Laboratory, Shanghai Research Institute of Stomatology, the Ninth
    People's Hospital, Shanghai Jiaotong University School of Medicine,
    Shanghai 200011, China)

Received date: 2011-08-12

  Online published: 2011-10-20

Supported by

the Science and Technology Commission Fund of Shanghai Municipality (No.09JC1411700), the Collaboration Projects of Development and Research from Basic Science of Stomatology of Shanghai (No. S30206-KF09), and the Fund of Shanghai Jiaotong University School of Medicine (No. 09XJ21030)

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Abstract

Abstract:  To explore the possible mechanism of osteogenesis for deciduous
teeth stem cells (DTSCs) in vivo/ vitro, stem cells from goat deciduous
teeth (SGDs) were firstly isolated, induced and transplanted into
immunocompromised mice. The SGDs's mineralization pattern and osteogenesis
were compared with bone marrow messenchymal stem cells (BMMSCs) from goats.
SGDs have similar osteogenic differentiation pattern in vitro and bone-like
tissue formation mechanism in vivo to BMMSCs; moreover SGDs have stronger
alkaline phosphatase (ALP) gene expression and osteopontin (OPN)
gene expression levels than BMMSCs; also SGDs can form more bone-like
tissues than BMMSCs when cell-scaffold compounds are transplanted into
immunocompromised mice. This pre-clinical study in a large-animal model
confirms that DTSCs may be an appropriate source of stem cells in repairing
bone defects with tissue engineering.

Key words： stem cells from goat deciduous teeth (SGDs); mineralization;
osteogenesis; bone marrow mesenchymal stem cells (BMMSCs); tissue
engineering

Cite this article

ZHAO Wei (赵 伟), LU Jia-yu (陆家瑜), HAO Yong-ming (郝永明), ZHANG Xiu-li (张秀丽) QU Xiao-hui (瞿晓辉), HUA Li (华 丽), CAO Chun- hua (曹春花), ZOU De-rong (邹德荣) . Comparison of Osteogenesis Between Two Kinds of Stem Cells from Goat
Combined Calcium Phosphate Cement in Tissue Engineering[J]. Journal of Shanghai Jiaotong University(Science), 2011 , 16(5) : 628 -635 . DOI: 10.1007/s12204-011-1200-x

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