Target-to-Background Separation for Spectral Unmixing in In-Vivo Fluorescence Imaging

doi:10.1007/s12204-014-1548-9

上海交通大学学报（英文版） ›› 2014, Vol. 19 ›› Issue (5): 600-611.doi: 10.1007/s12204-014-1548-9

Target-to-Background Separation for Spectral Unmixing in In-Vivo Fluorescence Imaging

ZHAO Yong (赵勇), HU Cheng (胡程), PENG Jin-liang*(彭金良), QIN Bin-jie* (秦斌杰)

(School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai 200240, China)

出版日期:2014-10-31 发布日期:2014-11-12
通讯作者: PENG Jin-liang(彭金良), QIN Bin-jie (秦斌杰) E-mail:pjl76@sjtu.edu.cn, bjqin@sjtu.edu.cn

Target-to-Background Separation for Spectral Unmixing in In-Vivo Fluorescence Imaging

ZHAO Yong (赵勇), HU Cheng (胡程), PENG Jin-liang*(彭金良), QIN Bin-jie* (秦斌杰)

(School of Biomedical Engineering, Shanghai Jiaotong University, Shanghai 200240, China)

Online:2014-10-31 Published:2014-11-12
Contact: PENG Jin-liang(彭金良), QIN Bin-jie (秦斌杰) E-mail:pjl76@sjtu.edu.cn, bjqin@sjtu.edu.cn

摘要/Abstract

摘要： We present a novel fluorescence spectral unmixing based on target-to-background separation preprocessing, which effectively separates the multi-target fluorescence from all background autofluorescence (BF) without any hardware-based BF acquisition and tissue specific BF estimation. Specifically, we first enhance the intrinsic accumulation contrast in target-to-background fluorescence using h-dome transformation; then separate multi-target fluorescence areas from the background in sparse multispectral data utilizing kernel maximum autocorrelation factor analysis; we further use fast marching-based image inpainting method to patch up the removed target fluorescence areas and reconstruct the multispectral BF; with the BF matrix being subtracted from the original data, the multi-target fluorophores are easily unmixed from the subtracted data using multivariate curve resolution-alternating least squares method. In two preliminary in-vivo experiments, the proposed method demonstrated excellent performance to unmix multi-target fluorescences while other state-of-art unmixing methods failed to get desired results.

关键词: fluorescence imaging, spectral unmixing, autofluorescence removal, target detection, kernel maximum autocorrelation factor, target-to-background separation

Abstract: We present a novel fluorescence spectral unmixing based on target-to-background separation preprocessing, which effectively separates the multi-target fluorescence from all background autofluorescence (BF) without any hardware-based BF acquisition and tissue specific BF estimation. Specifically, we first enhance the intrinsic accumulation contrast in target-to-background fluorescence using h-dome transformation; then separate multi-target fluorescence areas from the background in sparse multispectral data utilizing kernel maximum autocorrelation factor analysis; we further use fast marching-based image inpainting method to patch up the removed target fluorescence areas and reconstruct the multispectral BF; with the BF matrix being subtracted from the original data, the multi-target fluorophores are easily unmixed from the subtracted data using multivariate curve resolution-alternating least squares method. In two preliminary in-vivo experiments, the proposed method demonstrated excellent performance to unmix multi-target fluorescences while other state-of-art unmixing methods failed to get desired results.

Key words: fluorescence imaging, spectral unmixing, autofluorescence removal, target detection, kernel maximum autocorrelation factor, target-to-background separation

中图分类号:

Q 632

ZHAO Yong (赵勇), HU Cheng (胡程), PENG Jin-liang*(彭金良), QIN Bin-jie* (秦斌杰). Target-to-Background Separation for Spectral Unmixing in In-Vivo Fluorescence Imaging[J]. 上海交通大学学报（英文版）, 2014, 19(5): 600-611.

ZHAO Yong (赵勇), HU Cheng (胡程), PENG Jin-liang*(彭金良), QIN Bin-jie* (秦斌杰). Target-to-Background Separation for Spectral Unmixing in In-Vivo Fluorescence Imaging[J]. Journal of shanghai Jiaotong University (Science), 2014, 19(5): 600-611.

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Target-to-Background Separation for Spectral Unmixing in In-Vivo Fluorescence Imaging

Target-to-Background Separation for Spectral Unmixing in In-Vivo Fluorescence Imaging

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