［1］SHAO J W, XUE S, YU G L, et al. Smartphone-controlled optogenetically engineered cells enable semiautomatic glucose homeostasis in diabetic mice［J］. Science Translational Medicine, 2017, 9(387): eaal22989.
［2］DUEBER J E, WU G C, MALMIRCHEGINI G R, et al. Synthetic protein scaffolds provide modular control over metabolic flux［J］. Nature Biotechnology, 2009, 27(8): 753-759.
［3］SWOFFORD C A, VAN DESSEL N, FORBES N S. Quorum-sensing Salmonella selectively trigger protein expression within tumors［J］. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(11): 3457-3462.
［4］UTHOFF S, BROKER D, STEINBUCHEL A. Current state and perspectives of producing biodiesel-like compounds by biotechnology［J］. Microbial Biotechnology, 2009, 2(5): 551-565.
［5］LI H, CANN A F, LIAO J C. Biofuels: Biomolecular engineering fundamentals and advances［J］. Annual Review of Chemical and Biomolecular Engineering, 2010, 1: 19-36.
［6］SCHIRMER A, RUDE M A, LI X Z, et al. Microbial biosynthesis of alkanes［J］. Science, 2010, 329(5991): 559-562.
［7］NAWABI P, BAUER S, KYRPIDES N, et al. Engineering Escherichia coli for biodiesel production utilizing a bacterial fatty acid methyltransferase［J］. Applied and Environmental Microbiology, 2011, 77(22): 8052-8061.
［8］AKHTAR M K, TURNER N J, JONES P R. Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities［J］. Proceedings of the National Academy of Sciences of the United States of America, 2013, 110(1): 87-92.
［9］LENNEN R M, PFLEGER B F. Engineering Escherichia coli to synthesize free fatty acids［J］. Trends in Biotechnology, 2012, 30(12): 659-667.
［10］PRATHER K L, MARTIN C H. De novo biosynthetic pathways: Rational design of microbial chemical factories［J］. Current Opinion in Biotechnology, 2008, 19(5): 468-474.
［11］NA D, KIM T Y, LEE S Y. Construction and optimization of synthetic pathways in metabolic engineering［J］. Currrent Opinion in Microbiology, 2010, 13(3): 363-370.
［12］CHEN G T, INOUYE M. Role of the AGA/AGG codons, the rarest codons in global gene expression in Escherichia coli［J］. Genes & Development, 1994, 8(21): 2641-2652.
［13］KANAYA S, YAMADA Y, KINOUCHI M, et al. Codon usage and tRNA genes in eukaryotes: Correlation of codon usage diversity with translation efficiency and with CG-dinucleotide usage as assessed by multivariate analysis［J］. Journal of Molecular Evolution, 2001, 53(4/5): 290-298.
［14］LAKEY D L, VOLADRI R K, EDWARDS K M, et al. Enhanced production of recombinant Mycobacterium tuberculosis antigens in Escherichia coli by replacement of low-usage codons［J］. Infection and Immunity, 2000, 68(1): 233-238.
［15］STENSTROM C M, JIN H N, MAJOR L L, et al. Codon bias at the 3′-side of the initiation codon is correlated with translation initiation efficiency in Escherichia coli［J］. Gene, 2001, 263(1/2): 273-284.
［16］THANGADURAI C, SUTHAKARAN P, BARFAL P, et al. Rare codon priority and its position specificity at the 5′ of the gene modulates heterologous protein expression in Escherichia coli［J］. Biochemical and Biophysical Research Communications, 2008, 376(4): 647-652.
［17］ZHANG L Q, XUE P, ZHANG H J. Overexpression in Escherichia coli, purification and characterization of Thermoanaerobacter tengcongensis elongation factor G with multiple rare codons［J］. Protein and Peptide Letters, 2007, 14(8): 804-810.
［18］WANG Y, LI C Y, KHAN M R I, et al. An engineered rare codon device for optimization of metabolic pathways［J］. Scientific Reports, 2016, 6: 20608.
［19］YU X Y, LIU T G, ZHU F Y, et al. In vitro reconstitution and steady-state analysis of the fatty acid synthase from Escherichia coli［J］. Proceedings of the National Academy of Sciences of the United States of America, 2011, 108(46): 18643-18648.
［20］LEE H, DELOACHE W C, DUEBER J E. Spatial organization of enzymes for metabolic engineering［J］. Metabolic Engineering, 2012, 14(3): 242-251.
［21］BAEK J M, MAZUMDAR S, LEE S W, et al. Butyrate production in engineered Escherichia coli with synthetic scaffolds［J］. Biotechnology and Bioengineering, 2013, 110(10): 2790-2794.
［22］HIRAKAWA H, HAGA T, NAGAMUNE T. Artificial protein complexes for biocatalysis［J］. Topics in Catalysis, 2012, 55(16/17/18): 1124-1137.
［23］PROSCHEL M, DETSCH R, BOCCACCINI A R, et al. Engineering of metabolic pathways by artificial enzyme channels［J］. Frontiers in Bioengineering and Biotechnology, 2015, 3: 1-13.
［24］WANG Y, WU Y Q, SUO Y, et al. Clustering enzymes using E.coli inner cell membrane as scaffold in metabolic pathway［EB/OL］. (2017-12-25) ［2018-04-15］. https://doi.org/10.1101/230425.
［25］PAILLER J, AUCHER W, PIRES M, et al. Phosphatidylglycerol: Prolipoprotein Diacylglyceryl Transferase (Lgt) of Escherichia coli has seven transmembrane segments, and its essential residues are embedded in the membrane［J］. Journal of Bacteriology, 2012, 194(9): 2142-2151.
［26］SCHIERLE C F, BERKMEN M, HUBER D, et al. The DsbA signal sequence directs efficient, cotranslational export of passenger proteins to the Escherichia coli periplasm via the signal recognition particle pathway［J］. Journal of Bacteriology, 2003, 185(19): 5706-5713.
［27］SKRETAS G, GEORGIOU G. Simple genetic selection protocol for isolation of overexpressed genes that enhance accumulation of membrane-integrated human G protein-coupled receptors in Escherichia coli［J］. Applied and Environmental Microbiology, 2010, 76(17): 5852-5859.
［28］AFRIN S,KHAN M R I,ZHANG W Y, et al. Membrane-located expression of thioesterase from Acinetobacter baylyi enhances free fatty acid production with decreased toxicity in Synechocystis sp. PCC6803［J］. Frontiers in Microbiology, 2018, 9: 02842.
［29］GUO H S, FEI J F, XIE Q, et al. A chemical-regulated inducible RNAi system in plants［J］. The Plant Journal, 2003, 34(3): 383-392.
［30］KONERMANN S, BRIGHAM M D, TREVINO A, et al. Optical control of mammalian endogenous transcription and epigenetic states［J］. Nature, 2013, 500(7463): 472-476.
［31］OHLENDORF R, VIDAVSKI R R, ELDAR A, et al. From dusk till dawn: One-plasmid systems for light-regulated gene expression［J］. Journal of Molecular Biology, 2012, 416(4): 534-542.
［32］QI L S, LARSON M H, GILBERT L A, et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression［J］. Cell, 2013, 152(5): 1173-1183.
［33］WU H, WANG Y, WANG Y, et al. Quantitatively relating gene expression to light intensity via the serial connection of blue light sensor and CRISPRi［J］. ACS Synthetic Biology, 2014, 3(12): 979-982.
