采用纳米氧化硅客体子球和聚苯乙烯磁性主体母球设计并制备了一种新型的主客体组装结构复合微球，将抗人绒毛膜促性腺激素（hCG）抗体化学固载于组装微球表面，在流式免疫荧光检测平台中研究其免疫检测性能，并与传统的酶联免疫（ELISA）检测方法进行了比较.结果表明，主客体组装结构微球在流式免疫荧光检测中具有优异的检测性能，在微球用量极低的情况下仍然具有非常好的检测线性和检测重复性，并且空白限较传统的ELISA方法降低了6/7.
A new type host-guest structured bead-on-bead (BOB) particle was designed and synthesized via covalent assembling guest nano silica particles onto host magnetic polymer particles. Using BOB particles as biocarrier, anti-human chorionic gonadotrophin (hCG) antibody was covalently immobilized on the surface of BOB particles. Then, the immunofluorescent detection capability in recognition hCG antigen on flow cytometry was studied and compared with traditional enzyme-linked immuno sorbent assay (ELISA). The result showed that BOB particles presented remarkable immunofluorescent detection capability on flow cytometry. BOB particles also showed excellent linear detection than traditional ELISA detection in extremely low particles amount. Further more, the limit of blank (LOB) of immunofluorescent detection on flow cytometry was nearly 1/7 to traditional ELISA detection.
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