荧光检测器杂散光抑制研究

上海交通大学学报（自然版） ›› 2011, Vol. 45 ›› Issue (11): 1602-1606.

荧光检测器杂散光抑制研究

金玉希a，黄梅珍a，吴平a，王尧a，黄鹏飞b

（上海交通大学 a. 物理系光科学与工程研究中心； b. 上海交通大学电子信息与电气工程学院，上海 200240）

收稿日期:2010-12-14 出版日期:2011-11-30 发布日期:2011-11-30
基金资助:
国家自然科学基金资助项目（61178083）

Study of Stray Light Suppression in Fluorescence Detector

(a. Institute of Optics & Optical Engineering, Department of Physics, b. School of Electronic, Information and Electrical Engineering, Shanghai Jiaotong University, Shanghai 200240, China)

Received:2010-12-14 Online:2011-11-30 Published:2011-11-30

摘要/Abstract

摘要： 以液相色谱荧光检测器中的单色器为研究对象，分析了单色器杂散光的主要来源；通过ASAP软件建模，对单色器中杂散光的分布进行了模拟，提出了抑制杂散光的几种结构设计，包括光阑、叶片和挡板，研究比较了它们对杂散光的抑制效果；在单色器中同时加上这3种结构后，杂散光抑制比到达了80%以上.通过实验对模拟结果进行了验证，实验结果与模拟结果基本吻合.

关键词: 荧光检测器, 单色器, 杂散光, 叶片结构

Abstract: The stray light is one of the most key performances of spectrum instruments, especially when the light signal to be detected is very weak. The main source of stray light in two monochromators of fluorescence detector for high performance liquid chromatography (HPLC) was analyzed. Through the ASAP software modeling, the formation and distribution of stray light in monochromator were simulated, several structures for stray light suppression were presented and designed, including the apertures, vanes and light baffles, then their stray light suppression effects were estimated. When these three structures are installed in the monochromator simultaneously, the stray light suppression ratio can reach up to 80%. The simulation results are verified through experiments, and the experimental results and the simulation results agree well.

Key words: fluorescence detector, monochromator, stray light, vanes

中图分类号:

TH741

金玉希a，黄梅珍a，吴平a，王尧a，黄鹏飞b. 荧光检测器杂散光抑制研究[J]. 上海交通大学学报（自然版）, 2011, 45(11): 1602-1606.

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